Bio Flow Cytometry Compensation Transformation
CheckedThis skill automatically corrects for spectral overlap in flow cytometry data, ensuring accurate quantification of cell populations.
Install on your platform
Showing Claude Code. One command installs this skill into any of the agents below.
- 1
Run this in your project
terminal$ npx skills add gptomics/bioskills --skill bio-flow-cytometry-compensation-transformation --full-depth -a claude-code Uses the open-source
skillsCLI and needs Node.js. Add-gto install it for your user instead of this project.
What we know about this skill
Publisher
gptomics
Taken from the source listing. We haven't verified who controls this account.
Install target
Live repository
Not pinned. Installing fetches whatever the repository holds at the time you run the command.
Automated safety checks
This skill passed all 6 checks. We read its files at one commit and looked for the things that make a skill dangerous to run — not for whether it works.
Declares what it does: passed
SKILL.md parses and declares a name and a description.
SKILL.md exists, its frontmatter parses, and it declares a name and a description.
No undeclared network calls: passed
Makes no network calls.
Every host the skill dials — from its scripts, its config endpoints or the commands it tells the agent to run — is either well-known package and API infrastructure or declared in its own frontmatter. Links in documentation are not counted.
No obfuscated payloads: passed
No encoded or obfuscated payloads.
No base64, hex or compressed blob is decoded and then executed, and no encoded shell commands.
No credential access: passed
Reads no keys, tokens, keychains or credential files.
Neither the code nor the SKILL.md instructions read SSH keys, cloud credentials, keychains, token stores, browser cookie databases or dotfiles that hold secrets.
No pipe-to-shell installers: passed
No pipe-to-shell or download-and-run installers.
No `curl … | sh` pattern, in the code or in the instructions, that runs code fetched at run time.
Pinned to a commit: passed
Scanned gptomics/bioskills at d91ed3d563.
The exact upstream commit SHA that was scanned is recorded, so the result refers to specific bytes.
- Commit
- d91ed3d563
- Scanned
- 3 files, 246 lines
About this skill
What it does
This skill performs flow cytometry data compensation and transformation. It corrects for spectral overlap between fluorescent channels, allowing for accurate analysis of protein expression levels. The tool can also perform mathematical transformations (e.g., arcsinh) to improve data visualization and statistical analysis.
When to use it
- Analyzing fluorescence-activated cell sorting (FACS) data where multiple fluorochromes are used simultaneously.
- Preparing flow cytometry datasets for downstream statistical analyses or machine learning models.
- Standardizing flow cytometry data across different experiments or laboratories.
- Generating publication-quality figures from flow cytometry results.
Key capabilities
- Compensation matrix calculation
- Spectral overlap correction
- Mathematical transformations (e.g., arcsinh)
- Data normalization
Example prompts
- "Can you compensate this FCS file using the provided compensation matrix?"
- "Transform these flow cytometry data with an arcsinh transformation."
- “Apply a compensation matrix and then perform a logicle transformation on this dataset.”
Tips & gotchas
The accuracy of compensation relies heavily on accurate experimental controls (single-stained samples). Ensure your compensation matrix is properly generated for optimal results.